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BIOL 112 BIOL112 PopGen1_ProtocolandDatasheet – Texas A&M

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BIOL 112 BIOL112 PopGen1_ProtocolandDatasheet – Texas A&M

Population Genetics I Lab 1
Learning Objectives
1.Calculate allelic and genotypic frequencies in a population.
2.Determine the expected genotypic frequencies of a population.
3.Explain Hardy-Weinberg Equilibrium and why it is used in population genetics.
Lab Safety Notes
1.Closed-toe shoes are required
2.Goggles are required.
3.Gloves are required.
4.Do not touch the electrophoresis chamber while the power supply is turned on.
5.All tips that come into contact with blood/hemoglobin should be disposed of in thebiohazardous tip waste.
Lab Activity 1: Malaria and Sickle Cell Anemia

Description

  

BIOL 112 BIOL112 PopGen1_ProtocolandDatasheet – Texas A&M

Population Genetics I Lab 1
Learning Objectives
1.Calculate allelic and genotypic frequencies in a population.
2.Determine the expected genotypic frequencies of a population.
3.Explain Hardy-Weinberg Equilibrium and why it is used in population genetics.
Lab Safety Notes
1.Closed-toe shoes are required
2.Goggles are required.
3.Gloves are required.
4.Do not touch the electrophoresis chamber while the power supply is turned on.
5.All tips that come into contact with blood/hemoglobin should be disposed of in thebiohazardous tip waste.
Lab Activity 1: Malaria and Sickle Cell Anemia
The World Health Organization (WHO) has established that someone dies from malariaapproximately every 40 seconds. Since the highest mortality rate is in Africa, WHO hasdecided to concentrate the bulk of its control budget to Africa. However, Africa is a bigplace.
Your class has been asked to help establish where the eradication efforts shouldbe concentrated. Each lab bench will evaluate blood samples from one of three regionsin Africa.
You will compare the percentage of sickle-cell carriers for the regions and,based upon the correlation between the prevalence of the sickle-cell gene and malaria,make a recommendation as to where to spend the WHO funding to help the mostpeople.
The three regions are:Al-Khartum,Sudanand environs(population 5,274,000).Johannesburg, South Africaand environs (population 7,860,780).Kinshasa, Democratic Republic of the Congoand environs (population 7,273,900).
Each lab bench constitutes a research group that will examine blood samples using gelelectrophoresis. Recall from Biology 111 that gel electrophoresis uses an electricalcurrent to run DNA or protein samples through a gel matrix and separate mixedconstituents based on molecular size.
Name: _______________________Section: ______________________
1.Retrieve one gel tray, an electrophoresis chamber, and a well comb. Raise thegel tray end gates and tighten the set screws. If an end gate is missing, then usemasking or labeling tape to secure the end of the tray.
2.Use a hand protector and obtain a flask of hot 1.3% agarose from the incubator.Pour enough agarose (-35 ml) into the gel tray until the agarose is just below thecenter notch on the tray. Place a 12-well comb in the outer tray notches and let itset (-20 minutes).
3.Practice using a micropipettor while the gel sets:Obtain an agar plate from the refrigerator and place it in a culture dish.Pour enough water over it to just fill the wells and cover the plate. Thissimulates the conditions you will face when loading blood samples into thegel.Obtain a 2-20 µI micropipettor and observe the volume scale (Figure 1).
Locate the decimal point and adjust until the volume reads 12.0 µI. Pushthe tip of the micropipettor firmly into a sterile yellow pipette tip to seat the tip.

BIOL 112 BIOL112 PopGen1_ProtocolandDatasheet – Texas A&M

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BIOL 112 – Spring 2013 Register Now BIOL Lab 2 and 3 Questions Only.docx Textbook Exercises Campbell Biology image Campbell Biology Ch 1, Section 1.1 The Study of life reveals unifying themes., Exercise F1.4

Eukaryotes are larger and more complex than prokaryotes.Campbell Biology Ch 2, Section 2.1 Matter consists of chemical elements in pure form and in combinations called compunds, Exercise 1Table salt (NaCl) is a compound made of the elements sodium and chlorine.Campbell Biology Ch 3, Section 3.1

Polar covalent bonds in water molecules result in hydrogen bonding, Exercise F3.2Oxygen has greater electronegativity than hydrogen and it tends to pull shared electrons in a water…Campbell Biology Ch 4, Section 4.1 Organic chemistry is the study of carbon compunds, Exercise F4.2

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Biologists use microscopes and biochemistry to study cells, Exercise F6.3With a change in the cutting plane, different internal regions will be exposed.Campbell Biology Ch 7, Section 7.1 Cellular membranes are fluid mosaics of lipids and proteins, Exercise F7.2

BIOL 112 BIOL112 PopGen1_ProtocolandDatasheet – Texas A&M

The region that contains the phosphate group (in glycerol) is in contact with water.Campbell Biology Ch 8, Section 8.1 An organism’s metabolism transforms matter and energy, subject to the laws of thermodynamics, Exercise 1Passive transport mechanisms are thermodynamically favorable.Campbell Biology Ch 9, Section 9.1

Catabolic pathways yield energy by oxidizing organic fuels, Exercise F9.4The electrons present in the nicotinamide molecule determine the oxidized and reduced forms.Campbell Biology BIO LAB AND LECTURE Tests Questions & Answers Showing 1 to 8 of 12 View all Please refer to the attachment to answer this question.

This question was created from Deuterostomes_protocolanddatasheet_QV (1).docx. 1 2 3 4 5 Please refer to the attachment to answer this question. This question was created from Deuterostomes_protocolanddatasheet_QV (1).docx. 1 2 3 4 5

Which of the following is NOT a major difference between "Bryophytes" and ALL other land plant phyla? absence vs. presence of xylem and phloem 1 2 3 4 5 An individual fern sporophyte is able to produce far more spores than an individual moss sporophyte because, unlike the moss, the fern sporophyte has 1 2 3 4 5

BIOL 112 BIOL112 PopGen1_ProtocolandDatasheet – Texas A&M

Which of the following is NOT evidence supporting the hypothesis of the endosymbiotic evolution of mitochondria and chloroplasts? photosynthetic eukaryotes 1 2 3 4 5 Mutations that cause changes in development are thought to be important in the appearance of "evolutionary novelty" because ontogeny recapitulates 1 2 3 4 5

Which of the following is NOT considered to be an important correlate of the oxygen revolution? the accumulation of ozone screened out harmful ultraviolet 1 2 3 4 5 Most modern reptiles are grouped in the Order Squamata. The ancestors of these reptiles originated in the Mesozoic and gave rise to numerous and varied species 1 2 3 4 5

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